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KMID : 0352219950170020871
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Kyung Hee Dental Journal
1995 Volume.17 No. 2 p.871 ~ p.881
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EXPERIMENTAL STUDY OF THE COLLAGEN MATRIX CONTRACTION BY GINGIVAL FIBROBLAST AND PERIODONTAL LIGAMENT FIBROBLAST
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Abstract
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The purpose of this in vitro study was to evaluate contraction of the human gingival and periodontal ligament fibroblast in the collagen matrix.
The cell cultures were prepared using human gingiva and tooth.
The collagen gels were observed daily with an inverted microscope and their area was determined by measuring the diameters of the collagen gel.
The configurations of human gingival and periodontal ligament fibroblasts were done by SEM observation.
The results as follows;
1. The contraction was found to be proportional to seeding cell without seeding cells, the contraction was not occured.
2. Periodontal ligament fibroblast was shown more fast contraction than gingival fibroblast.
3. The collagen matrix was translucent initially, but with fully contraction, the structure become opaque.
4. The collagen matrix with fibroblast were aggregated to form bundles and reorganized collagen fiber.
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KEYWORD
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